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Capillary Liquid Chromatography- Mass SpectrometryWhat is Chromatography? Chromatography is the practiced term for a set of laboratory approaches for the insularity of changes (Solid/Liquid/Gas). The mixture is dissolved in a fluid which called the planetary signifier, which carries it through a structure holding another material known as the unmoving phase. The various constituents of the mixture transport at variant velocities, causing them to separate. The separation is mainly based on differential partitioning between the mobile and its stationary phases. Subtle differences in a compounds partition coefficient get out in differential retention time on the stationary phase and thus changing the separation (Tomer, et al., 1994). Chromatography stand be preparative or analytical. The basic purpose of preparative chromatography is to separate the components of a mixture for more advanced uses (and also it is a method of purification). Analytical chroma tography is done usually with smaller amounts of material and is for measuring the relative proportions of analytic in a mixture. The two are not mutually exclusive (Covey, et al., 1986). Basically chromatography methods can categorize based on several factors. by chromatographic issue shape1. Column chromatography2. Planner chromatography (Paper and Thin layer methods)Displacement Chromatographyby physical state of mobile phase1.Gas2.Liquidby separation mechanism1.Adsorption chromatography2.Ion exchange chromatography3.Size exclusion chromatography4. partitioning chromatography5.Affinity chromatographyFigure 1 Classification of Chromatography techniques. Liquid Chromatography Liquid chromatography (LC) is an analytical c... ... their mass / charge ratio (m/z). Several different techniques are visible(prenominal) for both ionization and ion analysis, resulting in many different catogories of mass spectrometers with different combinations of these two processes. In prac tice, some combinations are far more various than others and the following descriptions focus on the major types of ion sources and mass analyzers that used in LC-MS systems.Ion parentages1)Electrospray Ionization Source2)Atmospheric Pressure Chemical Ionization Source3)Atmospheric Pressure Photo IonizationMass Analyzers1)Quadruple Analyzers2)Time-of-flight Analyzers3)Ion snare Analyzers4)Hybrid AnalyzersApplications of Capillary liquid chromatography- mass spectrometryBiochemical natural covering or genetic disorders,Drug Monitoring and Toxicology,Vitamins and Related Metabolites,Analysis of steroid hormone Hormones